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| Single Cell Workflow |
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To make single cell analysis Simple, Sensitive, and Affordable®, we have developed easy to use workflows for single cell RT-PCR, PCR, qPCR and sequencing that integrate seamlessly with single cell deposition and optical QC. |
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| Oncology |
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The study of tumor stem cells or circulating tumor cells requires the molecular analysis of single cells, because every cell IS different, and cells are extremely rare. |
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| Immunology |
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Immunologists tackle single cell questions in basic science, virology, infectious disease, and cancer biology |
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| Forensics and paternity testing |
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AmpliGrid’s 1µL assay volume increases sensitivity of standard human identification kits while saving costly reagents |
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| Single Cell Gene Expression Analysis |
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Gene expression analysis has never been easier. Make use of the outstanding Beckman Coulter single cell analysis porfolio. |
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| Flow Cytometry |
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Our flow cytometry sample mixer, slide adaptor and user guides make it easy to add single cell molecular analysis to high throughput cell sorting |
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| qPCR |
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Pre-amplifying single cells with the AmpliGrid system followed by standard qPCR combines existing workflows with superior single cell sensitivity |
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| Other Applications |
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| Advalytix based in Germany offers two distinct product lines for Life Science Research:
The Ampligrid platform for Molecular analysis of Single Cell and 1 ul assays with simple workflow and Low copy number sensitivity.
The Booster line of mixing solution for small volume powered by SAW(Surface Accoustic Waves) for efficient,non invasive,gentle mixing for Hybridization applications,dramatically reducing incubation times.
Low Volume
Heterogeneous Assay
AmpliGrid AG480E
Activated Microliter Reaction Slide
The AmpliGrid AG480E is a superior amplification platform for low volume applications in the 1µL range, stabilized on a chemically structured microscope slide..
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Result Interpretation
AmpliGrid Automated Cell Detection
Cell Detection software module for Olympus cell package
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This innovative system offers sensitivity, efficiency and reproducibility for amplification-based tests such as ELISA.
Key Features:
- Flat glass slide with 48 chemically structured reaction sites
- Activated reaction sites to immobilize nucleic acids or proteins
- Replaces surface-coated microtiter plates (MTP) for heterogeneous assays
- Integrated lab on chip processing (i.e. lysis, amplification and detection via hybridization to capture probes)
- Fully automation compatible
Applications:
- Amplification-based-tests (i.e. infectious disease, oncology)
- ELISA: integrated immunoassay reaction plus detection
- Integrated custom chips (i.e. environmental microbiology)
Advantages:
- Economic: 25x reagent saving
- Sensitive: 2-10x more sensitive than same system in MTP
- Robust: Highly resistant to airborne contamination
- Easy to automate: Compatible with state of the art pipetting platforms
- Integration of assay and detection on a single slie
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Key Features:
- Automated image acquisition, cell detection and counting for research applications
- Add-on module for Olympus microscopes and software
- Multiple parameter options: cell size, fluorescence,
cell form factor
- Output: Text or Microsoft* Excel files for compatible data
processing
Applications:
- Single cell analysis
- High-throughput screening
- Routine flow cytometry cell sorting
Advantages:
- Complete and reliable experimental documentation
- Minimized hands-on time
- No subjective result interpretation
- Fast and convenient workflow
- Walk-away operation
http://www.advalytix.com/advalytix/ |
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